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clonepix2  (Danaher Inc)


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    Structured Review

    Danaher Inc clonepix2
    Clonepix2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clonepix2/ClonePix+2+Mammalian+Colony+Picker/us12559560-532-11-12
    Average 94 stars, based on 14 article reviews
    clonepix2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Expressing:

    Article Title: Overcoming Challenges of HIV Vaccine Antigen Production
    Article Snippet: Following transfection, cells were serially diluted from 500-5,000 cells/mL in semi- solid CHO-Growth A media with 1XHT and L-glutamine (Molecular Devices, Sunnyvale, CA), 500 μg/ml G418, and 10 μg/ml Alexa Fluor 488 conjugated goat antigp120 polyclonal antibodies reactive with clade B, AE, and C rgp120s as described previously [164]. .. Colonies expressing rgp120 were selected on basis of fluorescence and default selection parameters using the ClonePix2 (Molecular Devices, Sunnyvale, .. Cell culture in shake flasks was performed in BalanCD CHO Growth A medium (Irvine Scientific, Santa Ana, CA) supplemented with 1XHT (Corning, Corning NY), 10% ProyieldQ Cotton seed protein (DOMO, Netherlands), and 0.25 mM glutaMAX (Sigma-Aldrich Chemicals, St. Louis, MO).

    Fluorescence:

    Article Title: Overcoming Challenges of HIV Vaccine Antigen Production
    Article Snippet: Following transfection, cells were serially diluted from 500-5,000 cells/mL in semi- solid CHO-Growth A media with 1XHT and L-glutamine (Molecular Devices, Sunnyvale, CA), 500 μg/ml G418, and 10 μg/ml Alexa Fluor 488 conjugated goat antigp120 polyclonal antibodies reactive with clade B, AE, and C rgp120s as described previously [164]. .. Colonies expressing rgp120 were selected on basis of fluorescence and default selection parameters using the ClonePix2 (Molecular Devices, Sunnyvale, .. Cell culture in shake flasks was performed in BalanCD CHO Growth A medium (Irvine Scientific, Santa Ana, CA) supplemented with 1XHT (Corning, Corning NY), 10% ProyieldQ Cotton seed protein (DOMO, Netherlands), and 0.25 mM glutaMAX (Sigma-Aldrich Chemicals, St. Louis, MO).

    Selection:

    Article Title: Overcoming Challenges of HIV Vaccine Antigen Production
    Article Snippet: Following transfection, cells were serially diluted from 500-5,000 cells/mL in semi- solid CHO-Growth A media with 1XHT and L-glutamine (Molecular Devices, Sunnyvale, CA), 500 μg/ml G418, and 10 μg/ml Alexa Fluor 488 conjugated goat antigp120 polyclonal antibodies reactive with clade B, AE, and C rgp120s as described previously [164]. .. Colonies expressing rgp120 were selected on basis of fluorescence and default selection parameters using the ClonePix2 (Molecular Devices, Sunnyvale, .. Cell culture in shake flasks was performed in BalanCD CHO Growth A medium (Irvine Scientific, Santa Ana, CA) supplemented with 1XHT (Corning, Corning NY), 10% ProyieldQ Cotton seed protein (DOMO, Netherlands), and 0.25 mM glutaMAX (Sigma-Aldrich Chemicals, St. Louis, MO).

    Article Title: Overcoming Challenges of HIV Vaccine Antigen Production
    Article Snippet: .. To address these problems we combined a high efficiency electroporation device (MaxCyte STX, MaxCyte Inc., Gaithersburg, MD), a robotic cell selection system, ClonePix2 (Molecular Devices, Sunnyvale, CA) and a novel cell line (MGAT1- CHO 3.4F10) that was recently developed in our lab [132]. ..

    Isolation:

    Article Title: Developing selection strategy for CHO-K1 cell line that secretes scfv-Fc fusion antibodies using ClonePix2
    Article Snippet: .. Methylcellulose-based semi-solid medium used in conceived with the ClonePix2 (Molecular Devices, USA) device, was used for the isolation of the resulting clones. .. CloneMatrix concentrate (2,5X) (Molecular Devices, K8600, USA) is diluted to 1X with DMEM-F12 medium containing 10% FBS and supported by anti-Fc CloneDetectTM (antiHuman IgG (Fc) Specific, Fluorescein antibody, 10000U/ml) (Molecular Devices, K8205, USA) or anti-H+L CloneDetectTM (anti-Mouse IgG (H+L) Specific, Fluorescein antibody, 10000U) (Molecular Devices, K8220, USA).

    Article Title: Antibody-based compositions for targeting tropomyosin receptor kinase B (TrkB) isoforms
    Article Snippet: Following a 12+ week boosting protocol splenocytes were isolated from high titer mice and electrofused to a subclone of the P3x63-Ag8 myeloma cell line (BTX, Harvard Apparatus). .. Hybridomas secreting peptide specific antibody were identified and isolated using a ClonePix2 (Molecular Devices, CPII) colony picker. ..

    Clone Assay:

    Article Title: Developing selection strategy for CHO-K1 cell line that secretes scfv-Fc fusion antibodies using ClonePix2
    Article Snippet: .. Methylcellulose-based semi-solid medium used in conceived with the ClonePix2 (Molecular Devices, USA) device, was used for the isolation of the resulting clones. .. CloneMatrix concentrate (2,5X) (Molecular Devices, K8600, USA) is diluted to 1X with DMEM-F12 medium containing 10% FBS and supported by anti-Fc CloneDetectTM (antiHuman IgG (Fc) Specific, Fluorescein antibody, 10000U/ml) (Molecular Devices, K8205, USA) or anti-H+L CloneDetectTM (anti-Mouse IgG (H+L) Specific, Fluorescein antibody, 10000U) (Molecular Devices, K8220, USA).

    other:

    Article Title: Improvements to a GP120 Based Subunit Vaccine for HIV
    Article Snippet: Cell culture in shake flasks was performed in BalanCD® CHO Growth A medium (Irvine Scientific, Santa Ana, CA) supplemented with 1XHT (Corning, Corning NY), 10% Proyield® Cotton seed protein (DOMO, Netherlands), and 0.25mM glutaMAX (Sigma-Aldrich Chemicals, St. Louis, MO).

    Binding Assay:

    Article Title: Development of antibody-drug conjugates targeting L1CAM to treat metastatic cancer
    Article Snippet: .. Hybridomas were subcloned using the ClonePix2 (Molecular Devices), and clonal supernatants were tested for binding to human L1CAM by ELISA and flow cytometry. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Development of antibody-drug conjugates targeting L1CAM to treat metastatic cancer
    Article Snippet: .. Hybridomas were subcloned using the ClonePix2 (Molecular Devices), and clonal supernatants were tested for binding to human L1CAM by ELISA and flow cytometry. ..

    Flow Cytometry:

    Article Title: Development of antibody-drug conjugates targeting L1CAM to treat metastatic cancer
    Article Snippet: .. Hybridomas were subcloned using the ClonePix2 (Molecular Devices), and clonal supernatants were tested for binding to human L1CAM by ELISA and flow cytometry. ..

    Colony Assay:

    Article Title: Antibody-based compositions for targeting tropomyosin receptor kinase B (TrkB) isoforms
    Article Snippet: Following a 12+ week boosting protocol splenocytes were isolated from high titer mice and electrofused to a subclone of the P3x63-Ag8 myeloma cell line (BTX, Harvard Apparatus). .. Hybridomas secreting peptide specific antibody were identified and isolated using a ClonePix2 (Molecular Devices, CPII) colony picker. ..

    Electroporation:

    Article Title: Overcoming Challenges of HIV Vaccine Antigen Production
    Article Snippet: .. To address these problems we combined a high efficiency electroporation device (MaxCyte STX, MaxCyte Inc., Gaithersburg, MD), a robotic cell selection system, ClonePix2 (Molecular Devices, Sunnyvale, CA) and a novel cell line (MGAT1- CHO 3.4F10) that was recently developed in our lab [132]. ..



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